Michael Schleicher and coworkers
most recent publications:
Ablation of cyclase-associated protein 2 (CAP2) leads to cardiomyopathy.
Peche VS, Holak TA, Burgute BD, Kosmas K, Kale SP, Wunderlich FT, Elhamine F, Stehle R, Pfitzer G, Nohroudi K, Addicks K, Stöckigt F, Schrickel JW, Gallinger J, Schleicher M, Noegel AA.
Cell Mol Life Sci. 2012 Sep 4. [Epub ahead of print]
Abstract
Cyclase-associated proteins are highly conserved proteins that have a role in the regulation of actin dynamics. Higher eukaryotes have two isoforms, CAP1 and CAP2. To study the in vivo function of CAP2, we generated mice in which the CAP2 gene was inactivated by a gene-trap approach. Mutant mice showed a decrease in body weight and had a decreased survival rate. Further, they developed a severe cardiac defect marked by dilated cardiomyopathy (DCM) associated with drastic reduction in basal heart rate and prolongations in atrial and ventricular conduction times. Moreover, CAP2-deficient myofibrils exhibited reduced cooperativity of calcium-regulated force development. At the microscopic level, we observed disarrayed sarcomeres with development of fibrosis. We analyzed CAP2's role in actin assembly and found that it sequesters G-actin and efficiently fragments filaments. This activity resides completely in its WASP homology domain. Thus CAP2 is an essential component of the myocardial sarcomere and is essential for physiological functioning of the cardiac system, and a deficiency leads to DCM and various cardiac defects
Tumor suppressor Hippo/MST1 kinase mediates chemotaxis by regulating spreading and adhesion.
Artemenko Y, Batsios P, Borleis J, Gagnon Z, Lee J, Rohlfs M, Sanséau D, Willard SS, Schleicher M, Devreotes PN.
Proc Natl Acad Sci U S A. 2012 Aug 21;109(34):13632-13637. Epub 2012 Jul 30.
Abstract
Chemotaxis depends on a network of parallel pathways that coordinate cytoskeletal events to bias cell movement along a chemoattractant gradient. Using a forward genetic screen in Dictyostelium discoideum, we identified the Ste20 kinase KrsB, a homolog of tumor suppressors Hippo and MST1/2, as a negative regulator of cell spreading and substrate attachment. The excessive adhesion of krsB(-) cells reduced directional movement and prolonged the streaming phase of multicellular aggregation. These phenotypes depended on an intact kinase domain and phosphorylation of a conserved threonine (T176) within the activation loop. Chemoattractants triggered a rapid, transient autophosphorylation of T176 in a heterotrimeric G protein-dependent and PI3K- and TorC2-independent manner. The active phosphorylated form of KrsB acts to decrease adhesion to the substrate. Taken together these studies suggest that cycling between active and inactive forms of KrsB may provide the dynamic regulation of cell adhesion needed for proper cell migration and chemotaxis. KrsB interacts genetically with another D. discoideum Hippo/MST homolog, KrsA, but the two genes are not functionally redundant. These studies show that Hippo/MST proteins, like the tumor suppressor PTEN and oncogenes Ras and PI3K, play a key role in cell morphological events in addition to their role in regulating cell growth.
The Genome of the Foraminiferan Reticulomyxa filosa.
Glöckner G, Hülsmann N, Schleicher M, Noegel AA, Eichinger L, Gallinger C, Pawlowski J, Sierra R, Euteneuer U, Pillet L, Moustafa A, Platzer M, Groth M, Szafranski K, Schliwa M. Curr Biol. 2014 Jan 6;24(1):11-8. doi: 10.1016/j.cub.2013.11.027. Epub 2013 Dec 12.
The Diaphanous-related formin dDia1 is required for highly directional phototaxis and formation of properly sized fruiting bodies in Dictyostelium.
Winterhoff M, Junemann A, Nordholz B, Linkner J, Schleicher M, Faix J. Eur J Cell Biol. 2013 Nov 23. pii: S0171-9335(13)00081-2. doi: 10.1016/j.ejcb.2013.11.002. [Epub ahead of print]
The cytohesin paralog Sec7 of Dictyostelium discoideum is required for phagocytosis and cell motility. Müller R, Herr C, Sukumaran SK, Omosigho NN, Plomann M, Riyahi TY, Stumpf M, Swaminathan K, Tsangarides M, Yiannakou K, Blau-Wasser R, Gallinger C, Schleicher M, Kolanus W, Noegel AA. Cell Commun Signal. 2013 Aug 1;11:54. doi: 10.1186/1478-811X-11-54.
Ablation of cyclase-associated protein 2 (CAP2) leads to cardiomyopathy. Peche VS, Holak TA, Burgute BD, Kosmas K, Kale SP, Wunderlich FT, Elhamine F, Stehle R, Pfitzer G, Nohroudi K, Addicks K, Stöckigt F, Schrickel JW, Gallinger J, Schleicher M, Noegel AA. Cell Mol Life Sci. 2013 Feb;70(3):527-43. doi: 10.1007/s00018-012-1142-y. Epub 2012 Sep 4.
Sultana H, Neelakanta G, Rivero F, Blau-Wasser R, Schleicher M, Noegel AA.
BMC Dev Biol. 2012 Jan 12;12(1):3. [Epub ahead of print]
Molecular architecture of the Spire-actin nucleus and its implication for actin filament assembly
Tomasz Sitar*, Julia Gallinger*, Anna Ducka , Teemu Ikonen, Michael Wohlhoefler, Kurt Schmoller, Andreas Bausch, Peteranne Joel, Kathleen Trybus, Angelika Noegel, Michael Schleicher, Robert Huber, Tad Holak
*These authors contributed equally to the work
Proc Natl Acad Sci U S A. 2011 Dec 6;108(49):19575-80. Epub 2011 Nov 21.
Phylogeny-wide analysis of social amoeba genomes highlights ancient origins for complex intercellular communication.
Heidel AJ, Lawal HM, Felder M, Schilde C, Helps NR, Tunggal B, Rivero F, John U, Schleicher M, Eichinger L, Platzer M, Noegel AA, Schaap P, Glöckner G.
Genome Res. 2011 Nov;21(11):1882-91. Epub 2011 Jul 14.
Regulation of the actin cytoskeleton by an interaction of IQGAP related protein GAPA with filamin and cortexillin I.
Mondal S, Burgute B, Rieger D, Müller R, Rivero F, Faix J, Schleicher M, Noegel AA.
PLoS One. 2010 Nov 10;5(11):e15440.
Phylogeny-wide analysisof social amoeba genomes highlights ancient origins for complex intercellular communication.
Heidel, A. J., Lawal, H. M., Felder, M., Schilde, C., Helps, N. R., Tunggal, B., Rivero, F., John, U., Schleicher, M., Eichinger, L., Platzer, M., Noegel, A. A., Schaap, P., Glöckner, G. (2011).
Genome Research
Overexpression of myosin-IIB in the brain of a rat model of streptozotocin-induced diabetes
Calábria LK, da Cruz GC, Nascimento R, Carvalho WJ, de Gouveia NM, Alves FV, Furtado FB, Ishikawa-Ankerhold HC, de Sousa MV, Goulart LR, Espindola FS
J Neurol Sci. 303, 43-49, 2011first page of the publication
Please send an email message to: hellen.ishikawa-ankerhold@med.uni-muenchen.de
if you need the complete pdf file.
The NDR family kinase NdrA of Dictyostelium localizes to the centrosome and is required for efficient phagocytosis.
Kastner PM, Schleicher M, Müller-Taubenberger A.
Traffic. 2010 Dec 7. doi: 10.1111/j.1600-0854.2010.01147.x. [Epub ahead of print]
Regulation of the actin cytoskeleton by an interaction of IQGAP related protein GAPA with
filamin and cortexillin I.
Mondal, S., Burgute B., Rieger, D., Müller, R., Rivero, F., Faix J.,Schleicher, M. and Noegel, A.A..
PLoS ONE 2010 5(11), e15440.
Phospholipids regulate localization and activity of mDia1 formin.
Ramalingam N, Zhao H, Breitsprecher D, Lappalainen P, Faix J, Schleicher M.
Eur J Cell Biol. 2010 Oct;89(10):723-32.PMID: 20619927 [PubMed]
Redundant and unique roles of coronin proteins in Dictyostelium.
Shina MC, Müller-Taubenberger A, Unal C, Schleicher M, Steinert M, Eichinger L, Müller R, Blau-Wasser R, Glöckner G, Noegel AA.
Cell Mol Life Sci. 2010 Jul 18. [Epub ahead of print]PMID: 20640912 [PubMed - as supplied by publisher]
A cytohesin homolog in Dictyostelium amoebae.
Shina MC, Müller R, Blau-Wasser R, Glöckner G, Schleicher M, Eichinger L, Noegel AA, Kolanus W.
PLoS One. 2010 Feb 23;5(2):e9378.PMID: 20186335 [PubMed - in process]Free PMC ArticleFree textRelated citations
A Coronin7 homolog with functions in actin-driven processes.
Shina MC, Unal C, Eichinger L, Müller-Taubenberger A, Schleicher M, Steinert M, Noegel AA.
J Biol Chem. 2010 Mar 19;285(12):9249-61. Epub 2010 Jan 12.PMID: 20071332 [PubMed - indexed for MEDLINE]
Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation.
Anna M. Ducka, Peteranne Joel, Grzegorz M. Popowicz, Kathleen M. Trybus, Michael Schleicher, Angelika A. Noegel, Robert Huber, Tad A. Holak and Tomasz Sitar
Proc. Natl. Acad. Sci. USA June 29, 2010 vol.107 no. 26 11757-11762
CP250, a novel acidic coiled coil protein of the Dictyostelium centrosome, affects growth, chemotaxis, and the nuclear envelope.
Blau-Wasser, R., Euteneuer, U., Xiong, H., Gassen, B., Schleicher, M. and Noegel, A.A. (2009).
Mol. Biol. Cell 20, 4348-4361.
The actinome of Dictyostelium discoideum in comparison to actins and actin-related proteins from other organisms.The actinome of Dictyostelium discoideum in comparison to actins and actin-related proteins from other organisms.
Joseph, J.M., Fey, P., Ramalingam, N., Liu, X.I., Rohlfs, M., Noegel, A.A., Müller-Taubenberger, A., Glöckner, G. and Schleicher, M. (2008).
PLoS ONE 3(7), e2654
Actin: its cumbersome pilgrimage through cellular compartments.
Schleicher, M. and Jockusch, B.M. (2008).
Histochem. Cell Biol. 129, 695-704 (copyright owner: Springer Verlag). pdf-file:
The Ste20-like kinase SvkA of Dictyostelium discoideum is essential for late stages of cytokinesis.
Rohlfs, M., Arasada, R., Batsios, P., Janzen, J. and Schleicher, M. (2007).
J. Cell Sci. 120, 4345-4354.
CAP2, cyclase-associated protein 2, is a dual compartment protein.
Peche, V., Shekar, S., Leichter, M., Korte, H., Schröder, R., Schleicher, M., Holak, T.A., Clemen, C.S., Ramanath, Y.B., Pfitzer, G., Karakesisoglou, I. and Noegel, A.A. (2007).
Cell. Mol. Life Sci. 64, 2702-2715.
Profilin isoforms in Dictyostelium discoideum.
Arasada; R., Gloss, A., Tunggal, B., Joseph, J.M., Rieger, D., Mondal, S., Faix, J., Schleicher, M.* and Noegel, A.A. (2007).
Biochim. Biophys. Acta (Mol. Cell Res.) 1773, 631-641. (* corresponding author)
Yersinia protein kinase YopO is activated by a novel G-actin binding process.
Trasak, C., Zenner, G., Vogel, A., Yüksekdag, G., Rost, R., Haase, I., Fischer, M., Israel, L., Imhof, A., Linder, S., Schleicher, M. and Aepfelbacher, M. (2007).
J. Biol. Chem. 282, 2268-2277.
Filamin regulated F-actin assembly is essential for morphogenesis and controls phototaxis in Dictyostelium.
Khaire, N., Müller, R., Blau-Wasser, R., Eichinger, L., Schleicher, M., Rief, M., Holak, T.A. and Noegel, A.A. (2007). J. Biol. Chem. 282, 1948-1955.
Mechanism of oligomerisation of cyclase-associated protein from Dictyostelium discoideum in solution.
Yusof, A.M., Jaenicke, E., Pedersen, J.S., Noegel, A.A., Schleicher, M. and Hofmann, A. (2006).
J. Mol. Biol. 362, 1072-1081.
Characterization of the Ste20-like kinase Krs1 of Dictyostelium discoideum.
Arasada, R., Son, H., Ramalingam, N., Eichinger, L., Schleicher, M.* and Rohlfs, M. (2006).
Eur. J. Cell Biol. 85, 1059-1068 (* corresponding author)
Trix, a novel Rac guanine nucleotide exchange factor from Dictyostelium discoideum is an actin-binding protein and accumulates on endosomes.
Strehle, A., Schleicher, M. and Faix, J. (2006).
Eur. J. Cell Biol. 85, 1035-1045
The annexins of Dictyostelium.
Marko,M., Prabhu, Y., Müller, R., Blau-Wasser, R., Schleicher, M. and Noegel, A.A. (2006).
Eur. J. Cell Biol. 85, 1011-1022
The bundling activity of VASP is required for filopodium formation.
Schirenbeck, A., Arasada, R., Bretschneider, T., Stradal, T.E.B., Schleicher, M.* and Faix, J. (2006).
Proc. Natl. Acad. Sci. USA 103, 7694-7699.(* corresponding author).
Filamins: promiscuous organizers of the cytoskeleton.
Popowicz, G.M., Schleicher, M., Noegel, A.A. and Holak, T.A. (2006).
Trends Biochem. Sci. 206, 411-419.
The actin cytoskeleton.
Rajesh, A. and Schleicher, M. (2006).
Encyclopedic Reference of Genomics and Proteomics, Springer Verlag, Vol. 1, p. 5-8.
Dictyostelium transcriptional host cell response upon infection with Legionella.
Farbrother, P., Wagner C., Na, J., Tunggal, B., Morio T., Urushihara, H., Tanaka, Y., Schleicher, M., Steinert, M. and Eichinger, L. (2006).
Cellular Microbiology 8, 438-456.
The folding pathway of a fast-folding immunoglobulin domain revealed by single-molecule mechanical experiments.
Schwaiger, I., Schleicher, M., Noegel, A.A. and Rief, M. (2005).
EMBO Reports 6, 46-52.
Formins and VASPs may co-operate in the formation of filopodia.
Schirenbeck, A., Arasada, R., Bretschneider, T., Schleicher, M. and Faix, J. (2005).
Biochem. Soc. Trans. 33, 1256-1259.
Cyclase-associated protein is essential for the functioning of the endo-lysosomal system and provides a link to the actin cytoskeleton.
Sultana, H., Rivero, F., Blau-Wasser, R., Schwager, S., Balbo, A., Bozzaro, S., Schleicher, M. and Noegel, A.A. (2005).
Traffic 6, 930-946.
The Diaphanous-related formin dDia2 is required for the formation and maintenance of filopodia.
Schirenbeck, A., Bretschneider, T., Arasada, R., Schleicher, M. and Faix, J. (2005).
Nature Cell Biology 7, 619-625.
The genome of the social amoeba Dictyostelium discoideum.
Eichinger, L., Pachebat, J. A., Glöckner, G., Rajandream, M.-A., Sucgang, R.. Berriman, M., Song, J., Olsen, R., Szafranski, K., Xu, Q., Tunggal, B., Kummerfeld, S., Madera, M., Konfortov, B. A., Rivero, F., Bankier, A. T., Lehmann, R., Hamlin, N., Davies, R., Gaudet, P., Fey, P., Pilcher, K., Chen, G., Saunder, D., Sodergren, E., Davis, P., Kerhornou, A., Nie, X., Hall, N., Anjard, C., Hemphill, L., Bason, N., Farbrother, P., Desany, B., Just, E., Morio, T., Rost, R., Churcher, C., Cooper, J., Haydock, S., van Driessche, N. Cronin, A., Goodhead, I., Muzny, D., Mourier, T., Pain, A., Lu, M., Harper, D., Lindsay, R., Hauser, H., James, K., Quiles, M., Madan Babu, M., Saito, T., Buchrieser, C., Wardroper, A., Felder, M., Thangavelu, M., Johnson, D., Knights, A., Loulseged, H., Mungall, K., Oliver, K., Price, C., Quail, M. A., Urushihara, H., Hernandez, J., Rabbinowitsch, E., Steffen, D., Sanders, M., Ma, J., Kohara, Y., Sharp, S., Simmonds, M., Spiegler, S., Tivey, A., Sugano, S., White, B., Walker, D., Woodward, J., Winckler, T., Tanaka, Y., Shaulsky, G., Schleicher, M., Weinstock, G., Rosenthal, A., Cox, E. C., Chisholm, R. L., Gibbs, R., Loomis, W. F., Platzer, M., Kay, R. R., Williams, J., Dear, P. H., Noegel, A. A., Barrell, B., Kuspa, A. (2005).
Nature 435, 43-57.
Calnexin, calreticulin and cytoskeleton-associated proteins modulate uptake and growth of Legionella pneumophila in Dictyostelium discoideum.
Fajardo, M., Schleicher, M., Noegel, A., Bozzaro, S., Killinger, S., Heuner, K., Hacker, J. and Steinert, M. (2004).
Microbiology 150, 2825-2835.
Molecular structure of the rod domain of Dictyostelium filamin.
Popowicz, G.M., Müller, R., Noegel, A.A., Schleicher, M., Huber, R. and Holak, T.D. (2004).
J. Mol. Biol. 342, 1637-1646.
A rapid and efficient method to generate multiple gene disruptions in Dictyostelium discoideum using a single selectable marker and the Cre-loxP system.
Faix, J., Kreppel, L., Shaulsky, G., Schleicher, M. and Kimmel, A.R. (2004).
Nucleic Acids Res. 32, e143.
A novel partner for Dictyostelium filamin is an alpha-helical, developmentally regulated protein.
Knuth, M., Khaire, N., Kuspa, A., Lu, S. J., Schleicher, M., Noegel A. A. (2004).
J. Cell Sci. 117, 5013-5022.
NMR structural characterization of the N-terminal domain of the adenylyl cyclase-associated protein (CAP) from Dictyostelium discoideum.
Mavoungou, C., Israel, L., Rehm, T., Ksiazek, D., Krajewski, M., Popowicz, G., Noegel, A.A., Schleicher, M., and Holak, T.A. (2004).
J. Biomolecular NMR 29, 73-84.
A mechanical unfolding intermediate in an actin-crosslinking protein.
Schwaiger, I., Kardinal, A., Schleicher, M., Noegel, A.A., and Rief, M. (2004).
Nature Struct. Mol. Biol. 11, 81-85.
The cyclase associated protein CAP as regulator of cell polarity and cAMP signaling in Dictyostelium.
Noegel, A.A., Blau-Wasser, R., Sultana, H., Müller, R., Israel, L., Schleicher, M.*, Patel, H. and Weijer, C.J. (2004).
Mol. Biol. Cell 15, 934-945. (* corresponding author)
Function, expression and localization of annexin A7 in platelets and red blood cells: insights derived from an annexin A7 mutant mouse.
Herr, C., Clemen, C.S., Lehnert, G., Kutschkow, R., Picker, S.M., Gathof, B.S., Zamparelli, C., Schleicher, M. and Noegel, A.A. (2003).
BMC Biochemistry 4:8.
Structure of the N-terminal domain of the adenylyl cyclase associated protein (CAP) from Dictyostelium discoideum.
Ksiazek, D., Brandstetter, H., Israel, L., Bourenkov, G.P., Katchalova, G., Janssen, K.P., Bartunik, H.D., Noegel, A.A., Schleicher, M.*, and Holak, T.A.* (2003).
Structure 11, 1171-1178. (*corresponding authors)
Villidin, a novel WD-repeat and villin-related protein from Dictyostelium, is associated with membranes and the cytoskeleton.
Gloss, A., Rivero, F., Khaire, N., Müller, R., Loomis, W.F., Schleicher, M.* and Noegel, A.A. (2003).
Mol. Biol. Cell 14, 2716-2727. (* corresponding author)
Blue light perception in plants. Detection and characterization of a light-induced neutral flavin radical in a C450A mutant of phototropin.
Kay, C.W., Schleicher, E., Kuppig, A., Hofner, H., Rüdiger, W., Schleicher, M., Fischer, M., Bacher, A., Weber, S. and Richter, G. (2003).
J. Biol. Chem. 278, 10973-10982.
Crystallization of cyclase associated protein from Dictyostelium discoideum.
Hofmann, A., Hess, S., Noegel, A.A., Schleicher, M. and Wlodawer, A. (2002).
Acta Crystallogr. 58, 1858-1861.
High-resolution dissection of phagosome maturation reveals distinct membrane trafficking phases.
Gotthardt, D., Warnatz, H.J., Henschel, O., Bruckert, F., Schleicher, M. and Soldati, T. (2002).
Mol. Biol. Cell 13, 3508-3520.
Sequence-specific (1H, 15N, 13C) resonance assignment of the N-terminal domain of the cyclase-associated protein (CAP) from Dictyostelium discoideum.
Rehm, T., Mavoungou, C., Israel, L., Schleicher, M. and Holak, T.A. (2002).
J. Biomolecular NMR 23, 337-338.
Sequence and analysis of chromosome 2 of Dictyostelium discoideum.
Glöckner, G., Eichinger, L., Szafranski, K., Pachebat, J., Bankier, A., Dear, P., Lehmann, R., Baumgart, C., Parra, G., Abril, J.F., Guigó, R., Kumpf, K., Tunggal, B., The Dictyostelium Genome Sequencing Consortium (Barrell, B., deJong, P., Desany, B., Dingermann, T., Gerisch, G., Gibbs, R., Kay, R., Kuspa, A., Muzny, D., Philippsen, P., Rajandream, M.-A., Schleicher, M., Schuster, S., Sucgang, R., Williams, Winckler, T., J., Zeng, K., Zhu, B.),Cox, E., Quail, M.A., Platzer, M., André Rosenthal, A. and Noegel, A.A. (2002).
Nature 418, 79-85.
Loss of the F-actin binding and vesicle associated protein comitin leads to a phagocytosis defect.
Schreiner, T., Mohrs, M.R., Blau-Wasser, R., v. Krempelhuber, A., Steinert, M., Schleicher, M. and Noegel A.A. (2002).
Eukaryotic Cell 1, 906-914.
Various bacterial pathogens and symbionts infect the amoeba Dictyostelium discoideum.
Skriwan, C., Fajardo, M., Hägele, S., Horn, M., Wagner, M., Michel, R., Krohne, G., Schleicher, M., Hacker, J. and Steinert, M. (2002).
Int. J. Med. Microbiol. 291, 615-624.
Practical aspects of the 2D 15N-{1H}-NOE experiment.
Renner, C., Schleicher, M., Moroder, L. and Holak, T.A. (2002).
J. Biomolecular NMR 23, 23-33.
Villin-type headpiece domains show a wide range of F-actin binding affinities.
Vardar, D., Chishti, A.H.,, Frank, B.S., Luna, E.J., Noegel, A.A., Oh, S.W., Schleicher, M. and McKnight, C.J. (2002).
Cell Motil. Cytoskeleton 52, 9-21.
Interaction of Legionella pneumophila with Dictyostelium discoideum.
Steinert, M., Hägele, S., Skriwan, C., Grimm, D., Fajardo, M., Heuner, K., Schleicher, M., Hentschel, U., Ludwig, W., Marre, R. and Hacker, J. (2002).
"Legionella" - 5th International Conference on Legionella (eds. R. Marre, Y. Abu Kwaik, C. Bartlett, N.P. Cianciotto, B.S: Fields, M. Frosch, J. Hacker, P.C. Lück), ASM Press, Washington, pp. 161-164.
Characterization of CD36/LIMPII-homologues in Dictyostelium discoideum.
Janssen, K.-P., Rost, R., Eichinger, L. and Schleicher, M. (2001).
J. Biol. Chem. 276, 38899-38910.
Dictyostelium discoideum: a genetic model system for the study of professional phagocytes. Profilin, phosphoinositides and the lmp gene family in Dictyostelium.
Janssen, K.-P. and Schleicher, M. (2001).
Biochim. Biophys. Acta 1525, 228-233.
Filamins as integrators of cell mechanics and signalling.
Stossel, T.P., Condeelis, J., Cooley, L., Hartwig, J.H., Noegel, A., Schleicher, M. and Shapiro, S.S. (2001).
Nature Reviews Molecular Cell Biology 2, 138-145.
Mapping the functional surface of domain 2 in the gelsolin superfamiliy.
Puius, Y., Fedorov, E.V., Eichinger, L., Schleicher, M. and Almo, S.C. (2000).
Biochemistry 39, 5322-5331.
Dissection of functional domains by expression of point-mutated profilins in Dictyostelium mutants.
Lee, S.S., Karakesisoglou, I., Noegel, A.A., Rieger, D. and Schleicher, M. (2000).
Eur. J. Cell Biol. 79, 92-103..
Cloning and characterization of ß-COP from Dictyostelium discoideum.
Mohrs, M.R., Janssen, K.-P., Kreis, T., Noegel, A.A. and Schleicher, M. (2000).
Eur. J. Cell Biol. 79, 350-357.
Dictyostelium discoideum: a new host model system for intracellular pathogens of the genus Legionella.
Hägele, S., Köhler, R., Merkert, H., Schleicher, M., Hacker, J. and Steinert, M. (2000).
Cell. Microbiol. 2, 165-171.
The actin cytoskeleton of Dictyostelium: a story told by mutants (Commentary).
Noegel, A.A. and Schleicher, M. (2000).
J. Cell Sci. 113, 759-766.
Evidence for a genetic interaction between profilin and a lysosomal membrane protein (DdLIMP) in the regulation of endocytosis and endosomal membrane trafficking in Dictyostelium discoideum.
Temesvari, L., Zhang, L., Janssen, K.-P., Schleicher, M. and Cardelli, J.A. (1999).
Mol. Biol. Cell 11, 2019-2031.
Assessing the role of ASP56/CAP homologue of Dictyostelium discoideum and the requirements for subcellular localization.
Noegel, A.A., Rivero, F., Albrecht, R., Janssen, K.-P., Köhler, J., Parent, C.A. and Schleicher, M. (1999).
J. Cell Sci. 112, 3195-3203.
Molecular architecture of the rod domain of the Dictyostelium gelation factor (ABP120).
Fucini, P., Köppel, B., Schleicher, M., Lustig, A., Holak, T.A., Müller, R., Stewart, M. and Noegel, A.A. (1999).
J. Mol. Biol. 291, 1017-1023.
The crystal structure of the Physarum polycephalum actin-fragmin kinase: an atypical protein kinase with a specialized substrate-binding domain.
Steinbacher, S., Hof, P., Eichinger, L., Schleicher, M., Gettemans, J., Vandekerckhove, J., Huber, R. and Benz, J. (1999).
EMBO J. 18, 2923-2929.
Identification of a suppressor of the Dictyostelium profilin-minus phenotype as a CD36/LIMPII homologue.
Karakesisoglou, I., Janssen, K.-P., Eichinger, L., Noegel, A.A. and Schleicher, M. (1999).
J. Cell Biol. 145, 167-181.
Dictyostelium as a model system for studies of the actin cytoskeleton by molecular genetics.
Eichinger, L., Lee, S.S. and Schleicher, M. (1999).
Microscopy Research and Technique 47, 124-134.
Effects of single amino acid substitutions in the actin-binding site on the biological activity of bovine profilin I. J.
Schlüter, K., Schleicher, M. and Jockusch, B.M. (1998).
Cell Sci. 111, 3261-3273.
Importance of a flexible hinge near the motor domain in kinesin-driven motility.
Grummt, M., Woehlke, G., Henningsen, U., Fuchs, S., Schleicher, M. and Schliwa, M. (1998).
EMBO J. 17, 5536-5542.
Stress-induced Ste20-like protein kinase from Dictyostelium phosphorylates the actin-binding protein severin.
Eichinger, L., Bähler, M., Dietz, M., Eckerskorn, C. and Schleicher, M. (1998).
J. Biol. Chem. 273, 12952-12959.
Crystallization and preliminary X-ray diffraction characterization of a dimerizing fragment of the rod domain of the Dictyostelium gelation factor (ABP120).
Fucini, P., McCoy, A.J., Gomez-Ortiz, M., Schleicher, M., Noegel, A.A. and Stewart, M. (1997).
J. Struct. Biol., 128, 192-195
Actin binding proteins: role and regulation; in: "Dictyostelium - A model system for cell and developmental biology",
Noegel, A.A., Rivero, F., Fucini, P., Bracco, E., Janssen, K.-P. and Schleicher, M. (1997).
Y. Maeda, K. Inouye and I. Takeuchi, eds. Universal Academy Press, Inc. Tokyo, Japan. pp 33-42
A novel type of protein kinase phosphorylates actin in the actin-fragmin complex.
Eichinger, L., Bomblies, L., Vandekerckhove, J., Schleicher, M.* and Gettemans, J. (1996).
EMBO J. 15, 5547-5556, (* corresponding author).
Structure/function studies on the pH-dependent actin-binding protein hisactophilin in Dictyostelium mutants.
Stoeckelhuber, M., Noegel, A.A., Eckerskorn, C., Köhler, J., Rieger, D. and Schleicher, M. (1996).
J. Cell Sci. 109, 1825-1835.
Hisactophilin-mediated binding of actin to lipid lamellae: a neutron reflectivity study of protein membrane coupling.
Naumann, C., Dietrich, C., Behrisch, A., Bayerl, T., Schleicher, M., Bucknall, D. and Sackmann, E. (1996).
Biophys. J. 71, 811-823.
Dictyostelium discoideum contains a family of calmodulin-related EF-hand proteins that are developmentally regulated.
André, B., Noegel, A.A. and Schleicher, M. (1996).
FEBS Lett. 382, 198-202.
The role of the cortical cytoskeleton: F-actin crosslinking proteins protect against osmotic stress, ensure cell size, cell shape and motility, and contribute to phagocytosis and development.
Rivero, F., Köppel, B., Peracino, B., Bozzaro, S., Siegert, F., Weijer, C.J., Schleicher, M., Albrecht, R. and Noegel, A.A. (1996).
J. Cell Sci. 109, 2679-2691.
Plant profilins rescue the aberrant phenotype of profilin-deficient Dictyostelium cells.
Karakesisoglou, I., Schleicher, M., Gibbon, B.C. and Staiger, C.J. (1996).
Cell Motil. Cytoskel. 34, 36-47.
Identification of a cyclase-associated protein (CAP) homologue in Dictyostelium discoideum and characterization of its interaction with actin.
Gottwald, U., Brokamp, R., Karakesisoglou, I., Schleicher, M. and Noegel, A.A. (1996).
Mol. Biol. Cell 7, 261-272.
Linking microfilaments to intracellular membranes: The actin-binding and vesicle associated protein comitin exhibits a mannose-specific lectin activity.
Jung, E., Fucini, P., Stewart, M., Noegel, A.A. and Schleicher, M. (1996).
EMBO J. 15, 1238-1246.
Viscoelastic properties of F-actin solutions in the presence of normal and mutated actin-binding proteins.
Janssen, K.-P., Eichinger, L., Janmey, P., Noegel, A.A., Schliwa, M., Witke, W. and Schleicher, M. (1996).
Arch. Biochem. Biophys. 325, 183-189.
Coactosin interferes with the capping of actin filaments.
Röhrig, U., Gerisch, G., Morozowa, L., Schleicher, M. and Wegner, A. (1996).
FEBS Lett. 374, 284-286.
The actin-binding protein hisactophilin binds in vitro to partially charged membranes and mediates actin coupling to membranes.
Behrisch, A., Dietrich, C., Noegel, A.A., Schleicher, M. and Sackmann, E. (1995).
Biochemistry 34, 15182-15190.
Mechanical perturbation elicits a phenotypic difference between Dictyostelium wild-type cells and cytoskeletal mutants.
Eichinger, L., Köppel, B., Noegel, A.A., Schleicher, M.*, Schliwa, M., Weijer, K., Witke, W. and Janmey, P.A. (1996).
Biophysical J. 70, 1054-1060. (* corresponding author)
The in vivo role of annexin VII (synexin): Characterization of an annexin VII deficient Dictyostelium mutant indicates an involvement in Ca2+-regulated processes.
Döring, V., Veretout, F., Albrecht, R., Mühlbauer, B., Schlatterer, C., Schleicher, M. and Noegel, A.A. (1995).
J. Cell Sci. 108, 2065-2076.
Structure of severin domain 2 in solution.
Schnuchel, A., Wiltscheck, R., Eichinger, L., Schleicher, M.* and Holak, T.A.* (1995).
J. Mol. Biol. 247, 21-27. (* corresponding authors)
Structure/function studies on cytoskeletal proteins in Dictyostelium amoebae as a paradigm.
Schleicher, M., André, B., Andréoli, C., Eichinger, L., Haugwitz, M., Hofmann, A., Karakesisoglou, J., Stöckelhuber, M. and Noegel, A.A. (1995).
FEBS Lett. 369, 38-42.
Dictyostelium amoebae that lack G-actin sequestering profilins show defects in F-actin content, cytokinesis and development.
Haugwitz, M., Noegel, A.A., Karakesisoglou, J. and Schleicher, M. (1994).
Cell 79, 303-314.
Conformation of thymosin beta4 in water determined by NMR spectroscopy.
Czisch, M., Schleicher, M., Hörger, S., Voelter, W. and Holak, T.A. (1993).
Eur. J. Biochem. 218, 335-344.
The 100kDa F-actin capping protein of Dictyostelium amoebae is a villin prototype ('protovillin').
Hofmann, A., Noegel, A.A., Bomblies, L., Lottspeich, F. and Schleicher, M. (1993).
FEBS Lett. 328, 71-76.
The actin-binding protein comitin (p24) is a component of the Golgi apparatus.
Weiner, O.H., Murphy, J., Griffiths, G., Schleicher, M. and Noegel, A.A. (1993).
J. Cell Biol. 123, 23-34.
The heat shock cognate protein from Dictyostelium affects actin polymerization through interaction with the actin-binding protein cap32/34.
Haus, U., Trommler, P., Fisher, P.R., Hartmann, H., Lottspeich, F., Noegel, A.A. and Schleicher, M. (1993).
EMBO J. 12, 3763-3771.
The Ca2+-binding domains in non-muscle type alpha-actinin: biochemical and genetic analysis.
Witke, W., Hofmann, A., Köppel, B., Schleicher, M. and Noegel, A.A. (1993).
J. Cell Biol. 121, 599-606.
Homonuclear three-dimensional NOE-NOE nuclear magnetic resonance spectra for structure determination of proteins in solution.
Habazettl, J., Schleicher, M., Otlewski, J. and Holak, T.A. (1992).
J. Mol. Biol. 228, 156-169.
Characterization of actin- and lipid-binding domains in severin, a Ca2+-dependent F-actin fragmenting protein.
Eichinger, L. and Schleicher, M. (1992).
Biochemistry 31, 4779-4787.
Structure of hisactophilin is similar to interleukin-1ß and fibroblast growth factor.
Habazettl, J., Gondol, D., Wiltscheck, R., Otlewski, J., Schleicher, M.* and Holak, T.A.* (1992).
Nature 359, 855-858. (* corresponding authors)
Cap100, a new PIP2-regulated protein that caps actin filaments but does not nucleate actin-assembly.
Hofmann, A., Eichinger, L., André, E., Rieger, D. and Schleicher, M. (1992).
Cell Motil. Cytoskel. 23, 133-144.
Dynamics of the Dictyostelium cytoskeleton during chemotaxis.
Schleicher, M. and Noegel, A.A. (1992).
The New Biologist 4, 461-472.
F-actin capping by cap32/34 requires heterodimeric conformation and can be inhibited with PIP2.
Haus, U., Hartmann, H., Trommler, P., Noegel, A.A. and Schleicher, M. (1991).
Biochem. Biophys. Res. Commun. 181, 833 - 839.
Redundancy in the microfilament system: Abnormal development of Dictyostelium cells lacking two F-actin cross-linking proteins.
Witke, W., Schleicher, M. and Noegel, A.A. (1992).
Cell 68, 53 - 62.
Homonuclear three-dimensional NOE-NOE nuclear magnetic resonance spectroscopy of proteins in water.
Ross, A., Cieslar, C., Oschkinat, H., Schleicher, M. and Holak, T.A. (1991).
J. Magn. Res. 95, 567-573.
Dictyostelium discoideum contains two profilin isoforms that differ in structure and function.
Haugwitz, M., Noegel, A.A., Rieger, D., Lottspeich, F. and Schleicher, M. (1991).
J. Cell Sci. 100, 481 - 489.
Dictyostelium annexin VII (synexin): cDNA sequence and isolation of a gene disruption mutant.
Döring, V., Schleicher, M. and Noegel, A.A. (1991).
J. Biol. Chem. 266, 17509 - 17515.
Domain structure in actin-binding proteins: Expression and functional characterization of truncated severin.
Eichinger, L., Noegel, A.A. and Schleicher, M. (1991).
J. Cell Biol. 112, 665 - 676.
Purification and cDNA-derived sequence of adenylosuccinate synthetase from Dictyostelium discoideum.
Wiesmüller, L., Wittbrodt, J., Noegel, A.A. and Schleicher, M. (1991).
J. Biol. Chem. 266, 2480 - 2485.
Phenotypes of cells with cytoskeletal mutations.
Noegel, A.A. and Schleicher, M. (1991).
Curr. Opinion Cell Biol. 3, 18 - 26.
Expression and function of genetically engineered actin binding proteins in Dictyostelium. Current Topics in Membranes and Transport 38,
Witke, W., Schleicher, M., Einberger, H., Neubert, W.J. and Noegel, A.A. (1991).
207 - 215.
Non-muscle alpha-actinin is an EF-hand protein; in"Novel Calcium-binding Proteins - Fundamentals and Clinical Implications",
Witke, W., Noegel, A.A. and Schleicher, M. (1991).
C. Heizmann, ed., Springer Press, pp. 321 - 337.
Genetic alteration of proteins in actin-based motility systems.
Gerisch, G., Noegel, A.A. and Schleicher, M. (1991).
Ann. Rev. Physiol. 53, 607 - 628.
Replacement of threonine residues by serine and alanine in a phosphorylatable heavy chain fragment of Dictyostelium myosin II.
Lück-Vielmetter, D., Schleicher, M., Grabatin, B., Wippler, J. and Gerisch, G. (1990).
FEBS Lett. 269, 239 - 243.
Heterodimeric capping proteins constitute a highly conserved group of actin-binding proteins.
Hartmann, H., Schleicher, M. and Noegel, A.A.
(1990).Dev. Genetics 11, 369 - 376.
A Dictyostelium mutant lacking an F-actin crosslinking protein, the 120kD gelation factor.
Brink, M., Gerisch, G., Isenberg, G., Noegel, A.A., Segall, J.E., Wallraff, E. and Schleicher, M. (1990).
J. Cell Biol. 111, 1477-1489.
A protein with homology to the C-terminal repeat sequence of Octopus rhodopsin and synaptophysin is member of a multigene family in Dictyostelium discoideum.
Noegel, A.A., Gerisch, G., Lottspeich, F. and Schleicher, M. (1990).
FEBS Lett. 266, 118 - 122.
Membrane-enclosed crystals in Dictyostelium discoideum cells, consisting of developmentally regulated proteins with sequence similarities to known esterases.
Bomblies, L., Biegelmann, E., Döring, V., Gerisch, G., Krafft-Czepa, H., Noegel, A.A., Schleicher, M. and Humbel, B.M. (1990).
J. Cell Biol. 110, 669 - 679.
Severin is a gelsolin prototype.
Yin, H.L., Janmey, P.A. and Schleicher, M. (1990).
FEBS Lett. 264, 78 - 80.
cDNA-derived sequence of UMP-CMP kinase from Dictyostelium discoideum and expression of the enzyme in E. coli.
Wiesmüller, L., Noegel, A.A., Bârzu, O., Gerisch, G. and Schleicher, M. (1990).
J. Biol. Chem. 265, 6339 - 6345.
Ca2+-binding proteins as components of the cytoskeleton. In "Calcium binding proteins in normal and transformed cells",
Schleicher, M., Eichinger, L., Witke, W. and Noegel, A.A. (1990).
R. Pochet, D.E.M. Lawson and C.W. Heizmann, eds., Plenum Press, Vol. 269, 99 - 102.
Contraction based motility in Dictyostelium discoideum. In "Stimulus Response Coupling: The Role of Intracellular Calcium-binding Proteins",
Noegel, A.A. and Schleicher, M. (1990).
V.L.Smith and J.R. Dedman, eds., CRC Press, pp. 57 - 82.
The Dictyostelium gelation factor shares a putative actin-binding site with -actinin and dystrophin and also has a rod domain containing six 100-residue motifs that appear to have a cross-beta conformation.
Noegel, A.A., Rapp, S., Lottspeich, F., Schleicher, M. and Stewart, M. (1989).
J. Cell Biol. 109, 607 - 618.
Ca2+ independent F-actin capping proteins: Cap32/34, a capping protein from Dictyostelium discoideum, does not share sequence homologies with known actin-binding proteins.
Hartmann, H., Noegel, A.A., Eckerskorn, C., Rapp, S. and Schleicher, M. (1989).
J. Biol. Chem. 264, 12639-12647.
Alpha-actinin synthesis can be modulated by antisense probes and is autoregulated in non-muscle cells.
Schulze, H., Huckriede, A., Noegel, A.A., Schleicher, M. and Jockusch, B.M. (1989).
EMBO J. 8, 3587 - 3593.
An extended ubiquitin of Dictyostelium is located in the small ribosomal subunit.
Müller-Taubenberger, A., Graack, H.-R., Grohmann, L., Schleicher, M. and Gerisch, G. (1989).
J. Biol. Chem. 264, 5319-5322.
A Dictyostelium mutant deficient in severin, an F-actin fragmenting protein, shows normal motility and chemotaxis.
André, E., Brink, M., Gerisch, G., Isenberg, G., Noegel, A., Segall, J.E., Schleicher, M. and Wallraff, E. (1989).
J. Cell Biol. 108, 985-995.
Hisactophilin, a histidine-rich actin-binding protein from Dictyostelium discoideum.
Scheel, J., Ziegelbauer, K., Kupke, T., Humbel, B.M., Noegel, A.A., Gerisch, G. and Schleicher, M. (1989).
J. Biol. Chem. 264, 2832-2839.
Biological role of actin-binding proteins in Dictyostelium discoideum examined using genetic techniques.
Noegel, A.A., Leiting, B., Witke, W., Gurniak, C., Harloff, C., Hartmann, H., Wiesmüller, E. and Schleicher, M. (1989).
Cell Motil. Cytoskel. 14, 69-74.
Functional analysis of actin-binding proteins in vitro and in vivo. In "Cytoskeletal and Extracellular Proteins: Structure, Interactions and Assembly",
Noegel, A., Ziegelbauer, K., Hartmann, H. and Schleicher, M. (1989).
U. Aebi and J. Engel (eds), Springer Series in Biophysics, SpringerVerlag, Heidelberg, pp60-61.
Calcium- and actin-binding sites in F-actin crosslinking molecules from Dictyostelium discoideum. In "Cytoskeletal and Extracellular Proteins: Structure, Interactions and Assembly",
Schleicher, M. and Noegel, A. (1989).
U. Aebi and J. Engel (eds), Springer Series in Biophysics, Springer-Verlag, Heidelberg, pp62-64.
Construction and analysis of Dictyostelium mutants with defects in actin-binding proteins.
Schleicher, M., Wallraff, E., Gerisch, G. and Isenberg, G. (1988).
Protoplasma 2. Suppl., 22-26.
Severin, gelsolin and villin share a homologous sequence in regions presumed to contain F-actin severing domains.
André, E., Lottspeich, F., Schleicher, M. and Noegel, A. (1988).
J. Biol. Chem. 263, 722-727.
A Dictyostelium mutant with severe defects in alpha-actinin: its characterization using cDNA probes and monoclonal antibodies.
Schleicher, M., Noegel, A., Schwarz, T., Wallraff, E., Brink, M., Faix, J., Gerisch, G. and Isenberg, G. (1988).
J. Cell Sci. 90, 59-71.
Actin-binding proteins are conserved from slime mold to man.
Schleicher, M., André, E., Hartmann, H. and Noegel, A. (1988).
Dev. Genetics 9, 521-530.
Calcium-sensitive non-muscle alpha-actinin contains EF-hand structures and highly conserved regions.
Noegel, A., Witke, W. and Schleicher, M. (1987).
FEBS Lett. 221, 391-396.
cDNA clones coding for alpha-actinin of Dictyostelium discoideum.
Noegel, A., Witke, W. and Schleicher, M. (1986).
FEBS Lett. 204, 107-109.
Studies on the transformation, translation, and structure of alpha-actinin in Dictyostelium discoideum.
Witke, W., Schleicher, M., Lottspeich, F. and Noegel, A. (1986).
J. Cell Biol. 103, 969 - 975.
Selection of Dictyostelium mutants defective in cytoskeletal proteins: Use of a monoclonal antibody that binds to the ends of alpha-actinin rods.
Wallraff, E., Schleicher, M., Modersitzki, M., Rieger, D., Isenberg, G. and Gerisch, G. (1986).
EMBO J. 5, 61-67.
Direct photoaffinity labeling of soluble GTP-binding proteins in Dictyostelium discoideum.
Schleicher, M., Witke, W. and Isenberg, G. (1986).
FEBS Lett. 200, 156 - 160.
Actin/capping protein interactions: Molecular mechanisms and principles of regulation. In "Cell motility II: Mechanisms and regulation",
Isenberg, G., Hinssen, H., Knoerzer, W., Maruta, H., Ohnheiser, R. and Schleicher, M. (1985).
H. Ishikawa, S. Hatano and S. Sato, eds., Acad. Press Tokyo, pp. 163 - 173.
New F-actin binding proteins from Dictyostelium discoideum.
Schleicher, M., Gerisch, G. and Isenberg, G. (1984).
EMBO J. 3, 2095 - 2100.
Structural characterization of a higher plant calmodulin: Spinacea oleracea.
Lukas, T.J., Iverson, D.B., Schleicher, M. and Watterson, D.M. (1984).
Plant Physiol. 75, 788 - 795.
Isolation and characterization of calmodulin from the motile green alga Chlamydomonas reinhardtii.
Schleicher, M., Lukas, T.J. and Watterson, D.M. (1984).
Arch. Biochem. Biophys. 229, 33 - 42.
Analysis of differences between Coomassie blue stain and silver stain procedures in polyacrylamide gels: Conditions for the detection of calmodulin and troponin C.
Schleicher, M. and Watterson, D.M. (1983).
Anal. Biochem. 131, 312 - 317.
Further characterization of calmodulin from the monocotyledon barley (Hordeum vulgare),
Schleicher, M., Lukas, T.J. and Watterson, D.M. (1983).
Plant Physiol. 73, 666 - 670.
Analysis of suborganellar fractions from spinach and pea chloroplasts for calmodulin binding proteins.
Roberts, D.M., Zielinski, R.E., Schleicher, M. and Watterson, D.M. (1983).
J. Cell Biol. 97, 1644 - 1647.
Calmodulin phylogenetics: sequence comparisons of calmodulins from vertebrates, higher plants and slime mold. In "Calcium binding proteins",
Lukas, T.J., Burgess, W.H., Marshak, D.R., Schleicher, M. and Watterson, D.M. (1983).
B. de Bernard, G.L. Sottocasa, G. Sandri, E. Carafoli, A.N. Taylor, T.C.Vanaman and R.J.P. Williams, p. 179 - 180.
Comparative studies of calmodulins. In "Calcium and Cell Function",
Burgess, W.H., Schleicher, M., Van Eldik, L.J. and Watterson, D.M. (1983).
W.Y. Cheung, ed., Acad. Press, New York, pp. 209 - 261.
Towards a molecular and atomic anatomy of calmodulin and calmodulin binding proteins.
Watterson, D.M., Burgess, W.H., Lukas, T.J., Iverson, D., Marshak, D.R., Schleicher, M., Erickson, B.W., Fok, K.-F. and Van Eldik, L.J. (1983).
Adv. Cyclic Nuc. Res., Vol. 16, S.J. Strada and W.J. Thompson, eds., Raven Press, New York, pp. 205 - 226.
Calmodulin. In "The Cytoskeleton in Plant Growth and Development",
Schleicher, M., Iverson, D.B., Van Eldik, L.J. and Watterson, D.M. (1982).
C.W. Lloyd, ed., Acad. Press, New York, pp. 85 - 106.